Exosome-based delivery of super-repressor IκBα relieves sepsis-associated organ damage and mortality
The inflammatory responses in various disease models can be attenuated through introduction of super-repressor IκB (srIκB), which is the dominant active form of IκBα and can inhibit translocation of nuclear factor kappa-B (NF-κB) into the nucleus, even in the presence of proinflammatory stimulation. An optogenetically engineered exosome system (EXPLOR) that we previously developed was applied for loading a large amount of srIκB into exosomes in stably transfected cells. We showed that intraperitoneal injection of purified srIκB-loaded exosomes (Exo-srIκBs) attenuates mortality and systemic inflammation in septic mouse models.
Cyclic-recombinase-reporter mouse model to determine exosome communication and function during pregnancy
Paracrine signaling and communication via exosomes are not tested during pregnancy or parturition. This study was conducted to determine feto-maternal exosome trafficking and function during pregnancy. The following were observed in this study: exosomes can traffic between the fetus and the mother; maternal exosomes reaching the fetal tissues can produce functional changes; and fetal-specific exosomes can be isolated and characterized in maternal liquid biopsy specimens.View More
Mesenchymal Stem Cell-Derived Extracellular Vesicles as Therapeutics and as a Drug Delivery Platform
Stem cell-based therapy was first used to treat muscular dystrophies and has been studied intensively for its efficacy in various disease models, including myocardial infarction, kidney injuries, liver injuries, and cancers. In this review, we summarized the potential mechanisms underlying MSC-derived EVs therapy as a drug delivery platform. Additionally, based on currently published data, we predicted a potential therapeutic role of cargo proteins shuttled by EVs from MSCs.View More
Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein–protein interaction module
Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named ‘exosomes for protein loading via optically reversible protein–protein interactions’ (EXPLORs).View More